教育經曆:

1994 - 1998 , 博士後 , 藥理毒理專業 , 日本國立大阪大學 藥學部
1989 - 1992 , 理學博士 , 北京大學生命科學中心蛋白質工程研究室
1986 - 1989 , 理學碩士 , 病理生理專業 , 中國醫科大學
1978 - 1983 , 理學學士 , 醫療系 , 中國醫科大學

工作經曆:

2008 - 至今 , 副教授 , beat365官方网站
2007 - 2008 , 客座教授 , 美國得克薩斯大學醫學院
2005 - 2007 , 副教授 , beat365官方网站
2003 - 2004 , 訪問學者 , 瑞士日内瓦大學醫學院
1999 - 2003 , 副教授 , beat365官方网站
1993 - 1998 , 課程博士 , 日本大阪大學藥學部
1992 - 1993 , 助理研究員 , 中國科學院生物物理所
1983 - 1986 , 助理研究員 , 遼甯省基礎醫學研究所

社會服務工作:

2003 - 2007 , 基金評審 , 國家自然科學基金會生物化學專家組

榮譽獎勵:

負責的項目“金屬硫蛋白與抗衰老，神經生長因子與抗AD的作用 機制研究”獲得國家教育部頒發的科技進步二等獎 , 2004 獲獎證書号：2004-046；成果登記号：360-01-04-10010657-02
北京大學李政道基金筠政學者 蛋白質化學 指導教授稱号 , 2003
本人曾經以負責人身份承擔國家“九五”攻關項目（96－C02－01－09）”神經生長抑制因子-一種新型治療老年性癡呆症的可能性藥物”，并于2002年獲得國家教育部頒發的科技進步二等獎 , 2002 獲獎證書号：2001-027；成果登記号：360-01-10011352-07

雜志編輯:

reviewer , International Journal of Biochemistry & Cell Biology , 2008 - 2010 reviewer , Biochemical and Biophysical Research Communication , 2008 - 至今 reviewer , J. Molecular Biology , 2007 - 至今 審稿 , 中國科學通報 Chinese Science Bulletin , 1999 - 2005

執教課程:

生物化學與分子生物學進展 , 講課 , 北京大學第三教室407 , 2010.9
生物化學與分子生物學進展 , 講課 , 北京大學第三教室407 , 2010.9
生物化學與分子生物學進展 , 講課 , 北京大學第三教室407 , 2010.9
生物化學與分子生物學進展 , 講課 , 北京大學第三教室407 , 2010.9
生物化學與分子生物學進展 , 講課 , 北京大學第三教室407 , 2010.9
生物無機化學 , 主持 , 北京大學 , 2002
生物無機化學 , 講課 , 北京大學 , 2001

      本實驗室主要興趣在于：

神經生長抑制因子(GIF)是是神經系統中第一個被鑒定具有神經元生長抑制功能的蛋白質。1991年，Uchida等在研究阿爾茨海默病(Alzheimer’s Disease, AD)時從人腦組織中首次分離得到該物質。GIF特異分布于中樞神經系統，且其基因及蛋白結構與MT高度同源，屬于金屬硫蛋白(metallothionein, MT) 第三亞型I(MT-III)，GIF的下調被認為與AD的發生有着密切的關系。MT分子均由?-,?-結構域及一個保守的連接區組成。GIF 的?-結構域為其功能結構域，而β-結構域的CPCP區為其可能的生物學功能位點。然而， GIF是如何表現其生物學功能的？GIF與AD的關系又如何呢？這些問題都亟待進一步的研究。

金屬硫蛋白（Metallohinein, MT）是一類低分子量、富含半胱氨酸的金屬結合蛋白,其參與體内必需金屬元素的代謝、有毒重金屬解毒以及細胞應激等。MT表達最顯著的特征是能被重金屬離子、細胞因子、應激條件以及甾族激素等多種物質和條件誘導。水生動物體内的MT含量常作為評價水體重金屬污染的生物标識物，因此對于魚類MT含量測定及基因轉錄調控機制的研究将為MT在金屬污染的生物監測的應用提供了理論依據。我們建立了白鲫魚MT的純化鑒定的方法，并證明白鲫魚MT基因上遊調控區域存在多種轉錄調節元件，如金屬反應元件(MRE)、糖皮質激素反應元件(GRE)和抗氧化元件（ARE)等，分析其功能。

Research Description:

Neuronal growth inhibitory factor (GIF) was the first demonstrated as a neuronal growth inhibitor. It’s first isolated by Uchida (1991) from human brain while he studied in Alzheimer’s disease (AD). Based on its highly gene homologous characterization of the function and protein structure with Metallothionein(MT), GIF was named as brain-specific member of the metallothionein (MT) family, MT-III. GIF was drastically reduced at both protein and mRNA levels and down-regulated in AD brains were suggested to be closely related to the etiology of AD pathological process. GIF consists of ?, ?-domain and a constant linker. The GIF?-domain was proved to be the functional domain and CPCP region was considered to play a critical role in its biological activity. However, How can the ?-domain and the CPCP region be functional in GIF and how is GIF implicated to function in AD pathological process are need to be answered by study on.

Metallothioneins are a family of low molecular weight, cysteine-rich, metal-binding proteins, which play important roles in metal ion homeostasis, detoxification, and cytoprotection during the acute phase response. The most conspicuous feature of MT is its inducibility by a variety of agents and conditions, including heavy metal ions, cytokines, stress, and hormones. The MT quantitation as an indicator in aquatic creature and the analyzing of the genes promoter region can validate to value the pollution of waters. We set up the routine methods of purifying and determining MTs in crucian carp (Carassius cuvieri), demonstrated the exist of various cis-acting elements in the promoter region of MT gene, such as metal regulatory element (MRE), glucocorticoid responsive element (GRE), or antioxidant response element (ARE) etc, then analyzing them.

1. Da Huang, Jianrong Zhang, Yongchao Fu, Liming Lu, Yiming Xu , Lingyan Ma, Minxiang Li, Lingyuan Xiong1 , Hongwei Ren* , Quantitative Determination for Nerve Growth Factor Concentration in Commercial Product by SEC‐HPLC , Bioscience Methods , 2011 , 2: 1-5
2. Zhiguo Sun, Hongwei Ren(co-Firt Author) ,Yan Liu, Jessica L. Teeling and Jun Gu , Phosphorylation of RIG-I by casein kinase II inhibits its anti-viral response , J. Virology , 2011 , 85:1036-47
3. Feng Liu, Su Wu, Hongwei Ren , Jun Gu* , Klotho suppresses RIG-I mediated senescence-associated inflammation , Nature Cell Biology , 2011 , 13: 254-62
4. Xu L, Xiao N, Liu F, Ren HW and Gu J , Inhibition of RIG-I and MDA5-dependent antiviral response by gC1qR at mitochondria , Proc Natl Acad Sci USA , 2009 , 106(5):1530-1535
5. Pengfei He, Mingxu Xu, Hongwei Ren* , Cloning and functional characterization of 5'-upstream region of metallothionein-I gene from Crucian Carp (Carassius cuvieri) , International Journal Biochemistry & Cell Biology , 2007 , 39(4):832-41
6. Binyun Ma, Hongwei Ren*, Jianping Wu et al , Molecular Cloning of cDNA Endoding for MT-I/MT-II and Analyzing of the Protein Structure in Yark , China Biotechnology , 2007 , 25, 62~68
7. Mingming Chu, Zhen-quan Guo, Hong-wei Ren* , Development of ELISA for metallothionein-II allows determination of heavy metal pollution of fresh water , Frontiers in Bioscience , 2006 , 11, 2113-2122
8. Hongwei Ren*, Mingxu Xu, Pengfei He et al, , Cloning of crucian carp (Carassius cuvieri) metallothionein-II gene and characterization of its gene promoter region , Biochemical Biophysical Research Communications , 2006 , 342, 1297-1304
9. Mingming Chu, Zhen-quan Guo, Hong-wei Ren* , Development of ELISA for metallothionein-II allows determination of heavy metal pollution of fresh water , Frontiers in Bioscience , 2006 , 11, 2113-2122
10. Hongwei Ren*, Mingxu Xu, Pengfei He et al, , Cloning of crucian carp (Carassius cuvieri) metallothionein-II gene and characterization of its gene promoter region , Biochemical Biophysical Research Communications , 2006 , 342, 1297-1304
11. Liu Y.,Hongwei Ren., Ji QZ. & Ru BG , Attenuation of zinc-induced neuronal death by the interaction of growth inhibitory factor with Rab3a in rat hippocampal neurons , Neurosci Lett , 2004 , 358, 149-52
12. Hongwei Ren, Binggen Ru, et al. , Different Protective roles in vitro of α- and β-domain of Growth Inhibitory Factor (GIF) on Neuron Injuries Caused by Oxygen Free Radicals , Biochim. Biophy. Acta , 2001 , 1568, 129~134
13. Hongwei Ren, Ru Binggen , Application of Metallothionein (MT) Genes in Transgenic Aspect , Progress in Bioengineering , 2000 , 4(1); 34-39