個人介紹:

孔道春，beat365官方网站、北大-清華生命科學聯合中心、蛋白質工程與植物基因工程國家重點實驗室教授，973項目首席科學家，現任全國酵母協會會長，冷泉港亞洲會議組織者：DNA Metabolism, Genomic Stability and Disease；Yeast and Life Sciences。 孔道春實驗室的研究方向：真核細胞DNA複制起始、細胞調控維持DNA複制叉穩定、Checkpoint 調控、Chromsfork 調控、DNA同源重組、DNA單鍊和雙鍊斷裂修複、染色質結構形成等重要生物事件的分子機制。

教育經曆:

1998-2000　　博士後，The Memorial Sloan-Kettering Cancer Center
1995-1998　　博士後，Harvard Medical School, 生化與分子藥理系
1990-1994　　博士，Temple University Medical School，生化系　　
1986-1989　　碩士，中國科學院上海生物化學研究所
1979-1983　　學士，蘭州大學生物學系

工作經曆:

2005-今　　 beat365官方网站
2000-2005　　美國NIH
1989-1990　　國家醫藥總局，上海醫藥工業研究院
1983-1986　　衛生部蘭州生物制品研究所

社會服務工作:

組織冷泉港國際會議：DNA Metabolism, Genomic Stability and Disease 和 Yeast and Life Sciences　　

執教課程:

《DNA代謝、基因組穩定性、及疾病》，《生物化學基礎（CLS）》，《高級生物化學（CLS）》

      目前，孔道春實驗室的研究項目：
1. 真核細胞DNA複制起始機制：确定Chafer等新複制起始蛋白的生化性質和生物功能，建立體外DNA複制體系、闡明複制叉裡的生化反應，研究複制起始嚴格調控機制；
2. 細胞調控維持停頓DNA複制叉穩定的分子機制：闡明一些新發現的checkpoint靶蛋白的調控機制、确定這些調控在維持停頓DNA複制叉穩定、維持基因組穩定的作用；
3. 真核細胞DNA單雙鍊斷裂修複機制：闡明新發現的DNA斷裂修複蛋白作用機制，闡明乳腺癌易感基因BRCA1、BRCA2在DNA同源重組中的作用機制，确定PARP1的作用機制；
4）基本染色質結構形成機制：研究Chromsfork調控機制，研究基本染色質結構形成機制；
5）癌症藥物篩選。

1. Xizhou Li, Lu Wang, Xiaoqin Liu, Zeqi Zheng, Daochun Kong§. Cellular regulation and stability of DNA replication forks in eukaryotic cells. DNA Repair (Amst). 2022 Dec;120:103418. doi: 10.1016/j.dnarep.2022.103418. Epub 2022 Oct 10.

2. Sijie Liu, Xizhou Li, Xiaoqin Liu, Jingna Wang, Lingyan Li, Daochun Kong§. RNA polymerase III directly participates in DNA homologous recombination. Trends Cell Biol. 2022 Dec;32(12):988-995. doi: 10.1016/j.tcb.2022.06.007. Epub 2022 Jul 8.

3. Zizhi Tang, Ming Zeng, Xiaojun Wang, Chang Guo, Peng Yue, Xiaohu Zhang, Huiqiang Lou, Jun Chen, Dezhi Mu, Daochun Kong, Antony M Carr, Cong Liu. Synthetic lethality between TP53 and ENDOD1. Nat Commun. 2022 May 23;13(1):2861. doi: 10.1038/s41467-022-30311-w.

4. Sijie Liu & Daochun Kong§. A direct role of RNA polymerase III and RNA in DNA homologous recombination. Molecular & Cellular Oncology. 2021 Jul 1;DOI:10.1080/23723556.2021.1935173.

5. Sijie Liu, Xiaoqin Liu, Daochun Kong§. A Recently Discovered Essential Factor for DNA Homologous Recombination: RNA Polymerase III. Journal of Cell Science & Therapy. 2021 Jun 22;12(6):302.

6. Yang Liu#, Lu Wang#, Xin Xu, Yue Yuan, Bo Zhang, Zeyang Li, Yuchen Xie, Rui Yan, Zeqi Zheng, Jianguo Ji, Johanne M Murray, Antony M Carr, Daochun Kong§. The intra-S phase checkpoint directly regulates replication elongation to preserve the integrity of stalled replisomes. Proc Natl Acad Sci U S A. 2021 Jun 15;118(24):e2019183118.

7. Sijie Liu#, Yu Hua#, Jingan Wang#, Lingyan Li#, Junjie Yuan, Bo Zhang, Ziyang Wang, Jianguo Ji, Daochun Kong§. RNA polymerase III is required for the repair of DNA double-strand breaks by homologous recombination. Cell. 2021 Mar 4;184(5):1314-1329.e10. doi: 10.1016/j.cell.2021.01.048. Epub 2021 Feb 23.

8. Lihong Wu, Yu Hua, Feiran Chang, Daochun Kong§. Girdin: an essential component of pre-replicative complex in human cells. BioRxiv. doi: https://doi.org/10.1101/634626 Posted 2019.5.13

9. Gang Feng#, Yue Yuan#, Zeyang Li, Lu Wang, Bo Zhang, Jiechen Luo, Jianguo Ji, Daochun Kong§. Replication fork stalling elicits chromatin compaction for the stability of stalling replication forks. Proc Natl Acad Sci U S A. 2019 Jul 16;116(29):14563-14572. doi: 10.1073/pnas.1821475116. Epub 2019 Jul 1.

10. Fanli Zeng#, Yu Hua#, Xiaoqin Liu, Sijie Liu, Kejing Lao, Ze Zhang, Daochun Kong§. Gpn2 and Rba50 Directly Participate in the Assembly of the Rpb3 Subcomplex in the Biogenesis of RNA Polymerase II. Mol Cell Biol. 2018 Jun 14;38(13). pii: e00091-18. doi: 10.1128/MCB.00091-18. Print 2018 Jul 1.

11. Ling Guan*, Peng He*, Fang Yang*, Yuan Zhang*, Yunfei Hu*, Jienv Ding*, Yu Hua, Yi Zhang,Qiong Ye, Jiazhi Hu, Tao Wang§, Changwen Jin§, Daochun Kong§, Sap1 is a replication initiation factor essential for the assembly of pre-replicative complex in the fission yeast Schizosaccharomyces pombe. J Biol Chem. 2017 Apr 14;292(15):6056-6075. doi: 10.1074/jbc.M116.767806. Epub 2017 Feb 21.

12. Bochao Liu, Jiazhi Hu, Jingna Wang, Daochun Kong§, Direct Visualization of RNA-DNA Primer Removal from Okazaki Fragments Provides Support for Flap Cleavage and Exonucleolytic Pathways in Eukaryotic Cells. J Biol Chem. 2017 Mar 24;292(12):4777-4788. doi: 10.1074/jbc.M116.758599. Epub 2017 Feb 3.

13. Huimin Zhang, Yu Hua, Rui Li, Daochun Kong§, Cdc24 Is Essential for Long-range End Resection in the Repair of Double-stranded DNA Breaks. J Biol Chem. 2016 Nov 25;291(48):24961-24973.

14. Ming Zeng, Laifeng Ren, Ken`Ichi Mizuno, Konstantinos Nestoras, Haibin Wang, Zizhi Tang, Liandi Guo, Daochun Kong, Qiwen Hu, Qun He, Lilin Du, Antony M. Carr, and Cong Liu, CRL4(Wdr70) regulates H2B monoubiquitination and facilitates Exo1-dependent resection. Nat Commun. 2016 Apr 21;7:11364.

15. Lihong Wu, Yang Liu, Daochun Kong§, Mechanism of chromosomal DNA replication initiation and replication fork stabilization in eukaryotes. Sci China Life Sci. 2014 May;57(5):482-7. Review.

16. Jiazhi Hu, Lei Sun, Fenfen Shen, Yufei Chen, Yu Hua, Yang Liu, Mian Zhang, Yiren Hu, Qingsong Wang, Wei Xu, Fei Sun, Jianguo Ji, Johanne M. Murray, Antony M. Carr, and Daochun Kong§, The intra-S phase checkpoint targets Dna2 to prevent stalled replication forks from reversing. Cell, 2012 , 149(6):1221-32.