北京大學定量生物學中心

學術報告

題    目: Intestine-specific removal of DAF-2 nearly doubles lifespan in Caenorhabditis elegans with little fitness cost

報告人: 董夢秋

北京生命科學研究所高級研究員

時    間: 12月12日（周一）13:00-14:00

地    點: 線上報告

騰訊會議号: 935484552

https://meeting.tencent.com/dm/DjIhfXV2x9Ty

主持人: 韓敬東 教授

摘 要:

Twenty-nine years following the breakthrough discovery that a single-gene mutation of daf-2 doubles Caenorhabditis elegans lifespan, it remains unclear where this insulin/IGF-1 receptor gene is expressed and where it acts to regulate ageing. Using knock-in fluorescent reporters, we determined that daf-2 and its downstream transcription factor daf-16 are expressed ubiquitously. Using tissue-specific targeted protein degradation, we determined that intracellular DAF-2-to-DAF-16 signaling in the intestine plays a major role in lifespan regulation, while that in the hypodermis, neurons, and germline plays a minor role. Notably, intestine-specific loss of DAF-2 activates DAF-16 in and outside the intestine, causes almost no adverse effects on development and reproduction, and extends lifespan by 94% in a way that partly requires non-intestinal DAF-16. Consistent with intestine supplying nutrients to the entire body, evidence from this and other studies suggests that altered metabolism, particularly down-regulation of protein and RNA synthesis, mediates longevity by reduction of insulin/IGF-1 signaling.

報告人簡介：

董夢秋，北京生命科學研究所高級研究員，耶魯大學博士，斯克利普斯研究所博士後，2007年回國，在北京生命科學研究所工作至今。董夢秋課題組以線蟲為模型研究關于衰老的基礎生物學問題，同時開發基于質譜的蛋白質組學技術，在兩個領域都取得了突破性進展，相關文章發表在Nature、Nature Methods、Nature Communications、eLife、Aging Cell, Journal of Proteome Research、Analytical Chemistry 等期刊。課題組開發的交聯肽段質譜鑒定技術是輔助解析蛋白質結構或蛋白-蛋白相互作用的有效工具，被全球很多實驗室采用。在生物學方面，課題組目前的研究重點是闡明胰島素信号通路如何調節壽命，以及通過觀測線蟲衰老的生物學過程回答衰老是什麼、從什麼階段開始等基本問題。